PRODUCTS
Biomolecule imaging and analysis instrument
- NGS QC, repeat expansion, protein-DNA binding, and more
- 100bp to 500kbp+ DNA sizing
- Simple sample prep with no cold chain consumables
- Fast analysis time from 1-6 min per sample
The range of potential applications for PRECYSE is huge
Fast and easy workflow
- No cold chain reagents required saving time and cost
- No dealing with expiration dates and room temp equilibrations
- No dyes or gels required
- Automation friendly for use with 96 well plates and multi-plex EZchips
- PRECYSE EZchip scanning is self-aligning offering walkaway scanning and analysis of multiple samples.
Machine learning NGS QC image software analysis
2D/3D Sample Molecule Imaging
Software Molecule Tracing Analysis
Sample Sizing Analysis & Statistics
Frequently asked questions
(1.) PRECYSE directly images (versus indirect by electrophoresis) and measures 100’s of individual molecules
(2.) Direct imaging offers superior accuracy allowing researchers to:
- Generating sizing data
- Visualize secondary structures
- Visualize sample contaminants
- Observe other biomolecule phenomena such as protein binding.
(3.) HSAFM is more efficient:
- Minimal sample requirement (2 µL of 1-50 pg/µL sample)
- No special cold chain reagents, gels, dyes or sizing ladders needed by electrophoresis.
Evizia’s machine learning software identifies DNA molecule ends, traces the molecule backbone enabling highly accurate sizing.
For quantitative assessment of DNA samples, approximately 100+ molecules should be analyzed to generate an accurate sizing histogram representative of the sample. This varies based on the type of sample being analyzed (gDNA vs short read library).
For example, in the case of ultra-high molecular weight DNA (UHMW), a qualitative assessment of DNA integrity is rapidly achieved by observing larger intact DNA molecules (50 – 100 kbp+) in the sample. For shorter length DNA samples such as Illumina libraries, 500+ molecules are easily observed and accurately measured in 1-2 minutes to generate sizing histograms.
PRECYSE images and analyzes DNA ranging from ~100 bp to 500+ kbp. The software contains built-in analysis options depending on the type and expected size of DNA samples.
Importantly, AFM imaging is based on molecule size, thus proteins such as polymerases, helicases, and CAS9 binding to DNA have all been easily imaged by PRECYSE. RNA can be imaged as well and will be available with the next generation of EVIZIA’s automated software analysis and sizing for RNA samples.
PRECYSE is a desktop system measuring 18″ wide x 18″ tall x 24″ deep (45 cm x 45 cm x 61 cm). It weighs ~75 lbs (34 kg), not including the required tower PC and monitor.
PRECYSE has a built-in vibrational stability system. It should be placed on a stable bench in ambient lab conditions without any major vibrational systems nearby (large centrifuges for example).
No other special lab requirements are needed.
Small DNA sizing such as Illumina libraries can be scanned and analyzed in 1-2 minutes, while ultra-high molecular weight DNA (UHMW) gDNA samples can take 6-10 minutes depending on sizing analysis required. Different sizes and types of samples can be processed on a single EZchip, thus throughput can vary depending on the sample types.
Today, PRECYSE currently runs on an 8 plex EZchip consumable. A 16 plex and potentially 48+ plex are being developed. The EZchip is prepared in less than 10 minutes and does not require thawing or room temperature equilibration of cold chain reagents.
